Immobilization Of Catalase From Red Poppy On Chitosan Beads
نویسنده
چکیده
Catalase enzyme from red poppy (Papaver rhoeas) plant was immobilized covalently with glutaraldehyde onto chitosan particles. The changes in the kinetic properties of the immobilized and free red poppy catalase were determined. The Km values were found to be 26 and 33 mM for free and immobilized red poppy catalase on chitosan. Furthermore, various characteristics of free and immobilized red poppy catalase, such as the temperature profile, thermal stability, optimum pH, reusability and storage stability were evaluated. When comparing, free and immobilized enzyme, change was observed in optimum temperature from 30°C to 40°C, respectively. Immobilized enzyme showed 10°C increase in temperature. The optimum pH was found 7.0 for free and immobilized catalase. Immobilized catalase showed higher storage stabilities than free catalase. Thermal stability increased with immobilization. Free catalase lost all its activity within 5 days, whereas immobilized catalase lost 25% of its activity within 60 days at 25 °C. The remaining activity of the catalase was about 50% after 6 cycles of batch operation. Keywords— catalase, immobilization, chitosan
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